2HJ3

Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2005-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	82.840, 82.840, 160.813
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.228
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	11820
<I/σ(I)>	14.2
Completeness [%]	99.2	100
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	2.4	293	17% PEG 4000, 0.1M sodium acetate,0.5M ammonium sulfate, pH 2.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	2.4	293	17% PEG 4000, 0.1M sodium acetate,0.5M ammonium sulfate, pH 2.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K