2HIO
HISTONE OCTAMER (CHICKEN), CHROMOSOMAL PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 287 |
| Collection date | 1991-12 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.820, 118.820, 102.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.100 |
| R-factor | 0.255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 13542 |
| Completeness [%] | 80.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 2 (M) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 60 (%) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | EDTA | 5 (mM) | |
| 8 | 1 | reservoir | 2-mercaptoethanol | 1 (%) |
