2HIH

Crystal structure of Staphylococcus hyicus lipase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-08-30
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	73.310, 77.960, 169.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.710 - 2.860
R-factor	0.213
Rwork	0.208
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ji3
RMSD bond length	0.005
RMSD bond angle	0.786
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.710	2.930
High resolution limit [Å]	2.860	2.860
Number of reflections	22929
Completeness [%]	99.0	99.7
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NaCl, Na succinate, DMSO, isopropanol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K