2HIG

Crystal Structure of Phosphofructokinase apoenzyme from Trypanosoma brucei.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.488
Spacegroup name	P 21 21 2
Unit cell lengths	118.765, 92.497, 79.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.450 - 2.400
R-factor	0.201
Rwork	0.201
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	34288
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	298	well solution of 1.7-1.8 M sodium formate in 0.1 M sodium acetate buffer. Protein 4-5 mg/ml in 20 mM TEA buffer pH 8.0., pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K