2HIA
Crystal structure of TTHB049 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 42.557, 42.557, 156.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 1.600 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.23200 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1v7q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.247 |
| Number of reflections | 20090 | |
| <I/σ(I)> | 12.7 | 5.3 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 12.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.1 | 295 | PEG4000, MES, lithium chloride, 3-phospho glycerate, pH 6.1, microbatch, temperature 295K |
