2HI7
Crystal structure of DsbA-DsbB-ubiquinone complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-22 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.90000 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 165.500, 165.500, 65.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.700 |
R-factor | 0.34221 |
Rwork | 0.341 |
R-free | 0.36154 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 1.556 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.200 | 3.900 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.060 | 0.527 |
Number of reflections | 10332 | |
<I/σ(I)> | 18.7 | 3.8 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 6.9 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | JeffamimeED2001, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |