2HI7
Crystal structure of DsbA-DsbB-ubiquinone complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-22 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 165.500, 165.500, 65.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.700 |
| R-factor | 0.34221 |
| Rwork | 0.341 |
| R-free | 0.36154 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.556 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.200 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.060 | 0.527 |
| Number of reflections | 10332 | |
| <I/σ(I)> | 18.7 | 3.8 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | JeffamimeED2001, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
