2HI2

Crystal structure of native Neisseria gonorrhoeae Type IV pilin at 2.3 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1999-12-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.08
Spacegroup name	C 2 2 2
Unit cell lengths	119.754, 124.941, 26.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.240 - 2.300
R-factor	0.259
Rwork	0.259
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1AY2
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	8868
Completeness [%]	93.2	71.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	22% PEG 400 5% HEPTANE TRIOL 50 mM CHES, PH 8.0, VAPOR DIFFUSION, TEMPERATURE 298K