2HI1
The structure of a putative 4-hydroxythreonine-4-phosphate dehydrogenase from Salmonella typhimurium.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918, 0.97932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.660, 92.892, 101.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.26613 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.459 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 28553 | |
| <I/σ(I)> | 1.198 | |
| Completeness [%] | 90.6 | 64.1 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2M Na formate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
