2HHM
STRUCTURE OF INOSITOL MONOPHOSPHATASE, THE PUTATIVE TARGET OF LITHIUM THERAPY
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.200, 86.200, 154.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.600 * |
| R-factor | 0.166 |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 1.500 * |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.064 * |
| Total number of observations | 64631 * |
| Number of reflections | 19468 * |
| Completeness [%] | 93.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 64-68 (%sat) | ||
| 2 | 1 | reservoir | glutathione | 5 (mM) | |
| 3 | 1 | reservoir | 3 (mM) | ||
| 4 | 1 | reservoir | 2-6 (mM) | can be replaced with 5mM CaSO4 | |
| 5 | 1 | drop | inositol monophosphatase | 10 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 20 (mM) | |
| 7 | 1 | drop | EGTA | 1 (mM) | |
| 8 | 1 | drop | o-phenanthroline | 2 (mM) |
