2HHL
Crystal structure of the human small CTD phosphatase 3 isoform 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.74 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.419, 49.857, 179.747 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ta0 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 3.199 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.265 |
| Number of reflections | 47666 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 87.7 | 74 |
| Redundancy | 1.7 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.2M lithium sulfate monohydrate, 0.1M bis-tris, 25% w/v PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
