2HH7

Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97927
Spacegroup name	P 64 2 2
Unit cell lengths	91.059, 91.059, 46.779
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.550
Rwork	0.231
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.267
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.550	2.550
Number of reflections	3987
Completeness [%]	99.4	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	30% Peg4000,0.1Msodium citrate tribasic dihydrate, 0.2M ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	30% Peg4000,0.1Msodium citrate tribasic dihydrate, 0.2M ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K