2HGS
HUMAN GLUTATHIONE SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 84.260, 84.260, 197.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.28100 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.037 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.100 | 2.100 * |
| Rmerge | 0.057 | 0.197 * |
| Total number of observations | 201501 * | |
| Number of reflections | 40495 | |
| Completeness [%] | 95.9 | 95.9 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 22 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 10 | 1 | reservoir | PEG4000 | 9-12 (%) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | ATP | 0.1 (mM) | |
| 5 | 1 | drop | protein | 3-5 (mg/ml) | |
| 6 | 1 | reservoir | MES | 100 (mM) | |
| 7 | 1 | reservoir | GSH | 10 (mM) | |
| 8 | 1 | reservoir | EDTA | 5 (mM) | |
| 9 | 1 | reservoir | 0.1-0.2 (M) |
