2HG9
Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with tetrabrominated phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.554, 139.554, 184.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.360 - 2.450 |
| R-factor | 0.18035 |
| Rwork | 0.179 |
| R-free | 0.20914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Unpublished structure of reaction centre at 1.95A resolution |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.738 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.068 | 0.614 |
| Number of reflections | 76588 | |
| <I/σ(I)> | 18 | 3.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 8.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Potassium phosphate, LDAO, 1,2,3-heptanetriol, dioxane, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 16.0K |
