2HG0

Structure of the West Nile Virus envelope glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-08
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	89.599, 89.599, 154.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.264
Rwork	0.261
R-free	0.30700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oke 1ztx
RMSD bond length	0.007
RMSD bond angle	1.581
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.190
High resolution limit [Å]	3.000	3.000
Rmerge	0.069	0.466
Number of reflections	12820
<I/σ(I)>	17.7	2.28
Completeness [%]	98.3	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	11% PEG 8000, 0.1M Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K