2HFT

THE CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF HUMAN TISSUE FACTOR AT 1.7 ANGSTROMS RESOLUTION

Replaces:  1HFT

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	FUJI FILM
Wavelength(s)	0.908
Spacegroup name	P 41 21 2
Unit cell lengths	45.200, 45.200, 230.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.690
R-factor	0.204
R-free	0.25400
RMSD bond length	0.012
RMSD bond angle	1.840
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	1.690
Rmerge	0.058
Number of reflections	24199
Completeness [%]	97.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	100 *	drop contains 0.05 ml of protein solution and 0.01 ml of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	23 (mg/ml)
2	1	drop	sodium chloride	0.1 (M)
3	1	drop	PMSF	1 (mM)
4	1	drop	Tris-HCl	20 (mM)
5	1	reservoir	ammonium sulfate	50 (%sat)
6	1	reservoir	Tris-HCl	0.1 (M)