2HFO

Crystal Structures of the Synechocystis Photoreceptor Slr1694 Reveal Distinct Structural States Related to Signaling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	200
Detector technology	CCD
Collection date	2005-04-10
Detector	MAR CCD 165 mm
Wavelength(s)	0.82653
Spacegroup name	P 21 21 2
Unit cell lengths	119.424, 147.682, 96.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.100
R-factor	0.23928
Rwork	0.237
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.558
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.000
High resolution limit [Å]	2.100
Number of reflections	85961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.4 M NH4Ac, 0.1 M Bis-Tris (pH 6.5), 20% PEG3350 and 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K