2HF2
Domain shifting confirms monomeric structure of Escherichia sugar phosphatase SUPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.774, 111.295, 68.802 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.25188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rlm |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.323 |
| Number of reflections | 42397 | |
| <I/σ(I)> | 13.4 | 3.6 |
| Completeness [%] | 95.6 | 73.6 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 290 | 20% PEG8000, 0.1 M SODIUM PHOSPHATE-CITRATE, 10% GLYCEROL, pH 4.50, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
