2HEW

The X-ray crystal structure of murine OX40L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	P 63
Unit cell lengths	74.149, 74.149, 48.815
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.450
R-factor	0.16765
Rwork	0.165
R-free	0.19220
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.275
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	27220
<I/σ(I)>	11.9	3.2
Completeness [%]	100.0	100
Redundancy	14.3	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	0.1 M NaAcetate, 2M Ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	0.1 M NaAcetate, 2M Ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	0.1 M NaAcetate, 2M Ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K