2HEK
Crystal structure of O67745, a hypothetical protein from Aquifex aeolicus at 2.0 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.687, 144.413, 144.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.997 |
| R-factor | 0.17686 |
| Rwork | 0.175 |
| R-free | 0.22010 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.452 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.070 |
| High resolution limit [Å] | 1.997 | 1.997 |
| Rmerge | 0.520 | |
| Number of reflections | 76300 | |
| <I/σ(I)> | 21.4 | 2.5 |
| Completeness [%] | 95.7 | 95.7 |
| Redundancy | 4.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 10 mg/ml protein was mixed with 1:1 ratio with reservoir consisted of: 200 mM NaCl, 100 mM Phosphate-Citrate buffer, 10% PEG 3000, 100 mM NaBr, 5% Glycerol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
