2HEA

CONTRIBUTION OF WATER MOLECULES IN THE INTERIOR OF A PROTEIN TO THE CONFORMATIONAL STABILITY

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-09-04
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	56.990, 60.900, 33.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.156
Rwork	0.156
RMSD bond length	0.009
RMSD bond angle	1.560
Data reduction software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800
Rmerge	0.067	0.162 *
Total number of observations	29620 *
Number of reflections	9899
Completeness [%]	87.4	67.4 *
Redundancy	2.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	4.5 *	10 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		2.5 (M)
3	1	reservoir	acetate	20 (mM)