2HE9
Structure of the peptidylprolyl isomerase domain of the human NK-tumour recognition protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.753, 72.786, 73.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.000 |
| R-factor | 0.14889 |
| Rwork | 0.146 |
| R-free | 0.19752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZCX |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.515 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 23807 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Well solution: 21% Peg 3350, 0.25M potassium sulfate; Protein solution: 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM DTT, 15 mg/mL protein, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
