2HDW
Crystal structure of hypothetical protein PA2218 from Pseudomonas Aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Wavelength(s) | 0.979, 0.97934, 0.97911, 0.97166 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.264, 136.337, 48.134 |
| Unit cell angles | 90.00, 108.74, 90.00 |
Refinement procedure
| Resolution | 22.720 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 37451 | |
| Completeness [%] | 98.5 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG3350, 0.1M Bis-Tris, 0.1M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
