2HDA
Yes SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-30 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 46.009, 46.009, 90.431 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.860 - 1.900 |
| R-factor | 0.21619 |
| Rwork | 0.214 |
| R-free | 0.26969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fyn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.065 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.047 | 0.238 |
| Number of reflections | 5626 | |
| <I/σ(I)> | 28.37 | 5.19 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 10.08 | 4.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 288 | 1.6 M ammonium sulphate, 0.1 M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 15K, temperature 288K |
