2HCK
SRC FAMILY KINASE HCK-QUERCETIN COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.500, 93.300, 176.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 3.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.31100 |
| Structure solution method | MIRAS/MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.079 | 0.145 |
| Total number of observations | 385451 * | |
| Number of reflections | 24477 * | |
| <I/σ(I)> | 17.7 | 4.5 |
| Completeness [%] | 98.1 | 96.4 |
| Redundancy | 15.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 292 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | quercetin | 10 (mM) | or 2AMP-PMP |
| 3 | 1 | reservoir | calcium acetate | 150 (mM) | |
| 4 | 1 | reservoir | cacodylate | 100 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 7 (%(w/v)) | |
| 6 | 1 | reservoir | ehthylene glycol | 16 (%(v/v)) |
