2HB0

Crystal Structure of CfaE, the Adhesive Subunit of CFA/I Fimbria of Enterotoxigenic Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 62 2 2
Unit cell lengths	143.346, 143.346, 231.434
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.17874
Rwork	0.178
R-free	0.20076
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.519
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.080
Rmerge	0.082
Number of reflections	63021
Completeness [%]	74.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	288	10% PEGMME 550, 0.1M sodium HEPES, 1.4M sodium malonate, pH 7.0, VAPOR DIFFUSION, temperature 288K