2HAU

Apo-Human Serum Transferrin (Non-Glycosylated)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	103
Detector technology	CCD
Collection date	2006-04-27
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9350
Spacegroup name	P 21 21 21
Unit cell lengths	87.996, 102.159, 197.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.273
Rwork	0.273
R-free	0.32400
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.330
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.071
Number of reflections	47968
<I/σ(I)>		3.2
Completeness [%]	95.9	83.6
Redundancy	6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	15 mg/ml protein, 0.3 M ammonium citrate, 16-18% PEG 3350 , pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	15 mg/ml protein, 0.3 M ammonium citrate, 16-18% PEG 3350 , pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	15 mg/ml protein, 0.3 M ammonium citrate, 16-18% PEG 3350 , pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	15 mg/ml protein, 0.3 M ammonium citrate, 16-18% PEG 3350 , pH 6.5, VAPOR DIFFUSION, temperature 293K