2HAF

Crystal structure of a putative translation repressor from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	59.360, 59.360, 236.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.830 - 2.880
R-factor	0.241
Rwork	0.241
R-free	0.28800
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.880	2.880
Number of reflections	6206
<I/σ(I)>	16.8
Completeness [%]	98.8	98.5
Redundancy	6.1	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 1.4 M tri-sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 1.4 M tri-sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K