2H9K
Structure of Hen egg white lysozyme soaked with Ni-cyclam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.324, 77.324, 37.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.640 - 1.750 |
| R-factor | 0.18378 |
| Rwork | 0.181 |
| R-free | 0.24591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.881 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.640 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 11516 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | Acetate buffer, NaCl, pH 4.5, VAPOR DIFFUSION, temperature 277K, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
