2H9G
Crystal structure of phage derived Fab BdF1 with human Death Receptor 5 (DR5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.814, 61.389, 108.269 |
| Unit cell angles | 90.00, 101.17, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.320 |
| R-factor | 0.2338 |
| Rwork | 0.228 |
| R-free | 0.28164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chain S from 1d0g Chain AB from 1FVE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 54784 | |
| <I/σ(I)> | 6.7 | 2 |
| Completeness [%] | 97.1 | 97.3 |
| Redundancy | 3.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | Crystals from drops containing an equal volume of protein and well solution consisting of 20% PEG 3350, 0.2M Na2HPO4, 0.1 M Bis-Tris, pH 6.1-6.8. The crystals were cryo-protected with well solution supplemented with 20% PEG 200., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
