2H9C
Native Crystal Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 138 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 93.148, 93.148, 60.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.900 - 2.350 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.067 | 0.378 |
| Number of reflections | 11526 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | 0.1 M acetate buffer, 4.8 - 5.3 M ammonium nitrate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
