2H9A
Corrinoid Iron-Sulfur Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.030, 44.270, 100.660 |
Unit cell angles | 90.00, 118.63, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.207 |
Rwork | 0.203 |
R-free | 0.26000 |
Structure solution method | MIR |
RMSD bond length | 0.017 |
RMSD bond angle | 1.900 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 48659 | |
Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | PEG 3350, 1mM beta-mercaptoethanol, 100 mM KI, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |