2H9A
Corrinoid Iron-Sulfur Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.030, 44.270, 100.660 |
| Unit cell angles | 90.00, 118.63, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.203 |
| R-free | 0.26000 |
| Structure solution method | MIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 48659 | |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | PEG 3350, 1mM beta-mercaptoethanol, 100 mM KI, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
