2H8V

Structure of empty Pheromone Binding Protein ASP1 from the Honeybee Apis mellifera L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	C 2 2 21
Unit cell lengths	76.098, 85.997, 50.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.21818
Rwork	0.210
R-free	0.26625
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.368
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.667
High resolution limit [Å]	2.600	2.600
Number of reflections	4835
Completeness [%]	99.2	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	298	200 nL of a 40 mg/mL protein solution in Tris 10 mM, NaCl 25 mM, mixed with 100 nL of 2.2 M ammonium sulfate, 0.2 M ammonium iodide, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 298K