2H8Q
Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.934, 92.934, 431.593 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.000 |
| Rwork | 0.230 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7k |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.966 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR (2.5) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.450 |
| Number of reflections | 65283 | |
| <I/σ(I)> | 7.1 | 20.7 |
| Completeness [%] | 86.2 | 50.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M HEPES, 45%MPD dissolved in 1:1 ratio by volume of ~15mg/ml protein, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
