2H8N
Structure of a glutamine-rich domain from histone deacetylase 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.398, 60.451, 60.450 |
| Unit cell angles | 90.00, 108.74, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.600 |
| R-factor | 0.283 |
| Rwork | 0.281 |
| R-free | 0.30700 |
| Structure solution method | SAD |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.730 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 19701 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 291 | 0.825M LITHIUM SULFATE, 55mM HEPES PH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 7.50 |
