2H8K
Human Sulfotranferase SULT1C3 in complex with PAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.161, 108.717, 136.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| Rwork | 0.292 |
| R-free | 0.34240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ad1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.390 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.149 | 0.067 | 0.651 |
| Number of reflections | 13470 | 1430 | 1182 |
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 98.4 | ||
| Redundancy | 5.7 | 5.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 291 | PAP, 18% PEG-3350, 0.2M ammonium formate, BisTris, pH 6.9, vapor diffusion, sitting drop, temperature 291K |
