2H8C
Structure of RusA D70N in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-18 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.616, 59.483, 90.721 |
| Unit cell angles | 90.00, 101.59, 90.00 |
Refinement procedure
| Resolution | 44.460 - 3.100 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q8r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.922 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 12485 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 298 | 20% PEG 3350, 0.2M Sodium Fluoride, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.10 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | Sodium Fluoride | ||
| 3 | 1 | 2 | Sodium Fluoride |
