2H7Q
Cytochrome P450cam complexed with imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-02-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.6 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.667, 103.750, 106.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.500 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pDB code 1PHC |
| RMSD bond angle | 0.005 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.432 |
| Number of reflections | 63319 | |
| <I/σ(I)> | 11.6 | 2.74 |
| Completeness [%] | 98.7 | 96.8 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | 50mM Potassium phosphate pH 7.4, 250mM KCL, 50mM dithiothreitol, 36-52% ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
