2H7O

Crystal structure of the Rho-GTPase binding domain of YpkA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	90
Detector technology	CCD
Collection date	2004-11-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	60.009, 60.009, 402.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.880 - 2.000
R-factor	0.209
Rwork	0.208
R-free	0.23700
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.739
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.054
High resolution limit [Å]	2.000	2.000
Number of reflections	30187
<I/σ(I)>	33.1	3.2
Completeness [%]	98.5	90.29
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	276	100mM CAPs pH 10.5, 140mM-180mM NaCl, 16%-18% PEG1500 Micro Seeding, VAPOR DIFFUSION, HANGING DROP, temperature 276K