2H7H
Crystal structure of the JUN BZIP homodimer complexed with AP-1 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-07-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.258, 74.203, 58.303 |
| Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.22398 |
| Rwork | 0.218 |
| R-free | 0.27427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.558 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.056 | 0.300 |
| Number of reflections | 14887 | |
| <I/σ(I)> | 12.2 | 3.65 |
| Completeness [%] | 92.0 | 65.7 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 15% PEG 400, 50 mM HEPES, 160 mM AmFormate, 30 mM MgAcetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | AmFormate | ||
| 4 | 1 | 1 | MgAcetate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 400 | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | AmFormate | ||
| 9 | 1 | 2 | MgAcetate |
