2H6Q

Histone H3 recognition and presentation by the WDR5 module of the MLL1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-16
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	134.257, 46.336, 111.405
Unit cell angles	90.00, 116.72, 90.00

Refinement procedure

Resolution	33.200 - 1.870
R-factor	0.15348
Rwork	0.152
R-free	0.18464
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1erj
RMSD bond length	0.013
RMSD bond angle	1.373
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.200	1.950
High resolution limit [Å]	1.880	1.880
Rmerge	0.091	0.271
Number of reflections	46407
<I/σ(I)>	19.8	6.63
Completeness [%]	99.4	95.1
Redundancy	6.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				Crystals were grown by hanging drop vapour equilibration in Nextal plates as follows: 1 ul of 10 15 mg ml-1 protein solution (10 mM Tris HCl (pH 7.4), 50 mM NaCl, and 10 mM 2-mercaptoethanol) were mixed with 1 ul of well solution composed of 50 mM HEPES (pH 7.5), 100 mM potassium formate, and 10-20% (w/v) polyethylene glycol 3350 and equilibrated at room temperature overnight against 1 ml of well solution.