2H6O

Epstein Barr Virus Major Envelope Glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	200
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	110.009, 110.009, 146.129
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.500
R-factor	0.3385
Rwork	0.326
R-free	0.36840
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	0.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.800
High resolution limit [Å]	3.500	3.500
Number of reflections	12276
Completeness [%]	92.5	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	2.45 M Na/K phosphate pH 6.5, 95 mM MOPS pH 6.5, 0.19 M NaCl, 2.5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	2.45 M Na/K phosphate pH 6.5, 95 mM MOPS pH 6.5, 0.19 M NaCl, 2.5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K