2H6L
X-Ray Crystal Structure of the Metal-containing Protein AF0104 from Archaeoglobus fulgidus. Northeast Structural Genomics Consortium Target GR103.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.673, 47.714, 47.665 |
| Unit cell angles | 70.75, 70.81, 70.83 |
Refinement procedure
| Resolution | 19.440 - 2.000 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 46200 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 40% PEG200, 0.1M Na-acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
