2H6E
Crystal structure of the D-arabinose dehydrogenase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2827 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 84.019, 84.019, 194.979 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.800 |
| R-factor | 0.19674 |
| Rwork | 0.195 |
| R-free | 0.23216 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 32525 | |
| Completeness [%] | 99.1 | 93 |
| Redundancy | 12.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.55 | 293 | 4 mg/ml protein, 0.1 M HEPES-NaOH, 10% v/v Jeffamine-600, pH 8.55, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
