2H6C
Crystal structure of reduced CprK in absence of any ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.637, 50.030, 76.415 |
| Unit cell angles | 90.00, 105.54, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.900 |
| R-factor | 0.2354 |
| Rwork | 0.232 |
| R-free | 0.30723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h6b |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.075 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.056 | 0.257 |
| Number of reflections | 16387 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 8% PEG 3350, 115mM MgCl2, 10mM DTT, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
