2H5Y
Crystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.421 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.153, 172.138, 112.036 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.070 - 1.700 |
| R-factor | 0.16833 |
| Rwork | 0.167 |
| R-free | 0.20237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1amf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.752 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 72786 | |
| <I/σ(I)> | 26.6 | 3.3 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M lithium sulfate, 0.1M Tris-HCl, 30% PEG 4000 , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
