2H5X

RuvA from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	137.642, 137.642, 88.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.490 - 2.700
R-factor	0.233
Rwork	0.233
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1BVS
RMSD bond length	0.370
RMSD bond angle	3.400
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.106	0.512
Number of reflections	23937
<I/σ(I)>	14.1	2.7
Completeness [%]	99.9	100
Redundancy	7.8	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein stock was buffered in 20 mM Tris-HCl at pH 8.0 with 100 mM sodium chloride and 10 mM b-ME. 0.1 M sodium succinate at pH 5.5 and 1.7 M ammonium sulfate was used as a precipitant, VAPOR DIFFUSION, HANGING DROP, temperature 293K