2H5P
Crystal structure of mStrawberry at pH 9.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.596, 43.443, 61.362 |
| Unit cell angles | 90.00, 111.84, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.210 |
| R-factor | 0.1383 |
| Rwork | 0.138 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.250 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Number of reflections | 70282 | |
| Completeness [%] | 100.0 | 88.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 75mM Glycine pH 10.5, 1.1M Sodium Citrate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
