2H5N
Crystal Structure of Protein of Unknown Function PG1108 from Porphyromonas gingivalis W83
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.961, 84.826, 164.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.010 |
R-factor | 0.19094 |
Rwork | 0.188 |
R-free | 0.24365 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.621 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.095 | 0.466 |
Number of reflections | 35476 | |
<I/σ(I)> | 15.1 | 2 |
Completeness [%] | 99.5 | 97.5 |
Redundancy | 3.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 20% 1,4 butanediol, 0.1 imidazole pH 8.0, 0.1 M Zn(oAC)2, 0.1M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |