2H5A
Complex of the enzyme PMM/PGM with xylose 1-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.008, 74.551, 86.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.720 |
| R-factor | 0.17736 |
| Rwork | 0.176 |
| R-free | 0.21317 |
| Structure solution method | refinement |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.188 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.790 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.063 | 0.385 |
| Number of reflections | 46057 | |
| Completeness [%] | 96.1 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | Na, K tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
