2H4R
Crystal structure of wildtype MENT in the native conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-19 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.203, 74.022, 57.505 |
| Unit cell angles | 90.00, 95.42, 90.00 |
Refinement procedure
| Resolution | 18.960 - 2.700 |
| Rwork | 0.205 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2H4S |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.185 | 0.571 |
| Number of reflections | 10420 | |
| <I/σ(I)> | 4.4 | 2.4 |
| Completeness [%] | 97.1 | 95.8 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 20% PEG-4000, 0.1M sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
